鸡Perilipin1基因启动子的克隆及分析

doi:10.11843/j.issn.0366-6964.2016.02.006

Abstract

Abstract:

The purpose of this study was to analyze the structure and characteristics of promoter of the chicken Perilipin1 gene.A DNA fragment with 2 kb was cloned from the 5′-flanking region of Perilipin1，then sequenced and analyzed by bioinformatics methods.Subsequently，the luciferase reporter gene plasmids containing the full-length region of Perilipin1 gene promoter and its serial truncation mutations were constructed，and transfected into chicken embryonic fibroblast cell line (DF-1).Through detecting the level of luciferase activity，the core promoter region was determined.The result of bioinformatics analysis revealed that the promoter region of the chicken Perilipin1 gene had no typical TATA box and CpG islands，but many binding sites of transcription factors，such as TFIID，Sp1，AP2，PPAR，RXR，SREBP1，C/EBP，GATA，ER and KLF5.Luciferase reporter assays demonstrated that the chicken Perilipin1 gene promoter could significantly trigger the expression of the reporter gene (P<0.01).With the promoter fragment gradually truncated from the 5′ end，luciferase activity increased correspondingly，and the -360/-11 promoter fragment showed the strongest activity.Taken together，the promoter region from -360/-11 bp has the highest transcriptional activity，which could be the core regulatory region of the chicken Perilipin1 gene.

CLC Number:

S831.2

ZHOU Wei-nan，SHI Ming-xin，QIAO Shu-pei，SHI Hong-yan，WANG Yu-xiang. Promoter Cloning and Analysis of Chicken Perilipin1 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 249-259.

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Promoter Cloning and Analysis of Chicken Perilipin1 Gene

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